This assesses collateral circulation (ulnar artery) to ensure hand viability if the radial artery is compromised.

Step-by-Step Procedure
Step 1: Preparation & Safety
- Informed Consent: Explain the procedure, its purpose, and that it is more painful than a venipuncture. Obtain verbal or written consent.
- Gather Equipment: ABG syringe (pre-heparinized), needle (typically 22-25 gauge), alcohol and chlorhexidine swabs, 2×2 gauze, adhesive bandage, ice slurry (if transport delay > 10 min), personal protective equipment (PPE): gloves, face shield.
- Patient Position: Supine or semi-Fowler’s with wrist extended. Place a small towel roll or positioning device under the wrist for hyperextension (approx. 30-45 degrees). Ask the patient to relax their hand (“like a handshake”).
Step 2: Artery Palpation & Site Selection
- Palpate the radial artery (medial to the radial styloid) for maximum pulse point.
- Avoid areas with scars, lesions, or overlying infection.
- Note the angle and depth of the artery.
Step 3: Skin Cleaning & Anesthesia (if used)
- Clean the site vigorously with alcohol, then chlorhexidine (or per facility protocol). Allow to air dry.
- Local Anesthetic (Best Practice): Using a small needle (e.g., 27-30g), inject 0.5-1.0 mL of 1% lidocaine WITHOUT epinephrine intradermally/subcutaneously over the artery. Wait 60-90 seconds for effect. This greatly reduces pain.
Step 4: Artery Puncture
- Prepare Syringe: Hold the ABG syringe like a pencil. Remove the needle cap.
- Anchor the Artery: Using the non-dominant hand, palpate the pulse firmly with the index and middle fingers. The fingers should straddle the intended puncture site.
- Insert the Needle: Enter the skin at a 30-45 degree angle, bevel up, aiming directly for the point of maximum pulsation between your two anchoring fingers.
- Advance: A flash of bright red, pulsatile blood will appear in the syringe hub. The syringe will self-fill due to arterial pressure. Do NOT pull back the plunger.
- Collect Sample: Obtain 1-2 mL of blood (most syringes have a mark).
Step 5: Post-Puncture
- Withdraw & Apply Pressure: Quickly withdraw the needle and immediately apply firm, direct pressure with sterile gauze for at least 5 minutes (longer if coagulopathic).
- Expel Air & Mix: Immediately remove any tiny air bubbles, cap the syringe securely, and gently roll it between your palms to mix heparin. Do NOT shake.
- Label & Cool: Label the syringe at the bedside with patient info. Place it in the ice slurryimmediately if there’s any delay in analysis.
- Patient Care: Apply an adhesive bandage after ensuring bleeding has stopped. Instruct the patient to avoid using the wrist heavily for an hour.
Step 6: Sample Analysis & Documentation
- Transport to analyzer promptly (ideally within 10 minutes).
- Document: Time of collection, site used, patient’s temperature, respiratory rate, FiO2 (if on oxygen), any complications (e.g., hematoma).